How to use Porphobilinogen in a sentence
Further testing is necessary to determine whether the precursor present is porphobilinogen or urobilinogen-only porphobilinogen is indicative of AIP.
For example, to determine whether the Watson-Schwartz urine test is positive for porphobilinogen or urobilinogen, chloroform is added to the test tube.
Urobilinogen is slightly insoluble in water, while porphobilinogen tends to be water-soluble.
The porphobilinogen mixes more readily in water than chloroform, so if the water layer is pink (from the dye added to the urine sample), that indicates the presence of porphobilinogen, and a diagnosis of AIP is probable.
Certain enzymes, such as porphobilinogen deaminase (the defective enzyme in AIP), can be easily extracted from red blood cells; other enzymes, however, are less readily collected or tested.Advertisement