Another method of genetic modification, called mutagenesis, dates to the early part of the twentieth century.
mutagenesis of the proteins can be used to identify amino acids that are important in appropriate targeting in health and disease.
Site directed mutagenesis has been used to generate such mutants.
We use site-directed mutagenesis to couple this information to structural features of the proteins.
Cloning, mutagenesis and expression The domains were amplified from cDNA using polymerase chain reaction (PCR ).
Our present study clearly demonstrates that anticancer chemotherapy can induce mutagenesis in vivo in various pediatric cancer patients.
A schematic illustration of insertional mutagenesis with one of our gene trap vectors.
We are using molecular genetic approaches, in particular random mutagenesis, to analyze fungal genes involved in the disease process.
A ' marker switch ' approach for targeted mutagenesis of genes in Schizosaccharomyces pombe.
For this we are using molecular modeling to investigate docking of the novel compounds, as well as conventional mutagenesis and expression techniques.
mutagenesis experiments coupled with biochemical studies to understand better the functioning of the enzyme.
mutagenesis mosaic screen.
mutagenesis studies, we are able to determine the actual sites of phosphorylation chosen by specific kinases.
mutagenesis programs is the production of mouse models of inherited human disease.
mutagenesis strategy was co-ordinated with acquisition of the DNA sequence of the PAI.
The mutants were generated by random transposon mutagenesis with the Tn pho A transposon.
The insertion mutagenesis strategy was co-ordinated with acquisition of the DNA sequence of the PAI.
The theory and application of in vitro mutagenesis, transgenic animals, and DNA fingerprinting.
Using this technique in combination with another mutagenesis method called saturation mutagenesis the ee value has been improved to more than 90 per cent.
specificity of an enzyme through mutagenesis.
transposon mutagenesis and sequencing was tested after sub-cloning of open reading frames into broad host range shuttle vectors.
One approach is to change the natural substrate specificity of an enzyme through mutagenesis.
The function of genes located by transposon mutagenesis and sequencing was tested after sub-cloning of open reading frames into broad host range shuttle vectors.
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