Lister determined the number of bacteria present in a drop of the liquid under examination by counting, and then diluted this with a sufficient quantity of sterilized water so that each drop of the mixture should contain, on an average, less than one bacterium.
Hansen counted the number of yeast cells suspended in a drop of liquid diluted with sterilized water.
A volume of the diluted yeast was introduced into flasks containing sterilized wort, the degree of dilution being such that only a small proportion of the flasks became infected.
But even filters of this type, if they are to be fully relied upon, must be frequently cleaned and sterilized, and great care must be taken that the joints and connexions are watertight, and that the candles are without cracks or flaws.
Schuppan has shown that it is possible to produce good butter from Pasteurized or even sterilized cream, and Weigmann introduced the plan of artificially souring cream by means of pure cultures of B.
Later Bonnier (1886) succeeded in producing fertile thalli by sowing lichen spores and the appropriate algae upon sterile glass plates or portions of bark, and growing them in sterilized air (fig.
It is especially desirable for hospital and ambulance staffs to be inoculated with a vaccine prepared from sterilized cultures of plague bacillus.
At Hubli, where nearly the whole population was inoculated between the lath of May and the 27th of September ' The system of inoculation against plague with a fluid prepared from sterilized virus of the disease was introduced in India by Professor Haffkine early in 1897.
This fluid was sterilized by methods approved by the Indian Plague Commission and contained the requisite proportion of carbolic acid.
Though nearly all its ancient abodes have been drained, and for its purposes sterilized these many years past, not a spring comes but it shows itself in small companies in the eastern counties of England, evidently seeking a breedingplace.
This toxin-containing broth is injected into a horse in increasing doses, just as in the case of the serpent venom, and after the resistance of the horse has been much increased it is bled into sterilized vessels and the blood is allowed to coagulate.
In the same way sterilized cultures of typhoid bacilli have been used to protect against attacks of typhoid fever, and an anti-typhoid serum has been employed with intent to cure.
It has been found possible to grow pure cultures of various diatoms, and by feeding these to delicate larvae kept in sterilized sea-water, great successes have been attained.
They showed that, when grown on sterilized sand with the addition of mineral salts, the Leguminosae were no more able to use the atmospheric nitrogen than other plants such as oats and barley.
Even when the light is not sufficiently intense, or the exposure is too short to kill the spores, the experiments show that attenuation of virulence, That bacterial fermentations are accompanied by the evolution of heat is an old experience; but the discovery that the " spontaneous " combustion of sterilized cotton-waste does not occur simply if moist and freely exposed to oxygen, philous bacteria.
In cultivating bacteria outside the body various media to serve as food material must be prepared and sterilized by heat.
The media from the store-flasks are placed in glass test-tubes or small flasks, protected from contamination by cotton-wool plugs, and are sterilized by heat.
For example, various sugars - lactose, glucose, saccharose, &c. - are added to test the fermentative action of the bacterium on these substances; litmus is added to show changes in reaction, specially standardized media being used for estimating such changes; peptone solution is commonly employed for testing whether or not the bacterium forms indol; sterilized milk is used as a culture medium to determine whether or not it is curdled by the growth.