With another similar protoplast, which constitutes what we call fertilization, the next stage in complexity already noted may be observed, the protoplasm becoming clothed by a cell-membrane.
This is a primal necessity of the protoplast,and every cell gives evidence of its need by adopting one of the various ways in which such need is supplied.
No doubt the primary object of the cell-wall of even the humblest protoplast is protection, and this too is the meaning of the coarser tegumentary structures of a bulkier plant.
Every protoplast, except in the very young regions, has part of its surface abutting on these, so that its wall is accessible to the gases necessary for its vital processes.
It does not at first appear to be the same with the bulkier plants, such as the ordinary green herbs, shrubs or trees, but a study of their earlier development indicates that they do not at the outset differ in any way from the simple undifferentiated forms. Each commences its existence as a simple naked protoplast, in the embroyo-sac or the archegonium, as the case may be.
If the access of oxygen to a protoplast is interfered with its normal respiration soon ceases, but frequently other changes supervene.
(4) The cell must have a certain temperature, for the activity of a protoplast is only possible within certain limits, which differ in the case of different plants.
(5) There must be a supply of oxygen to the growing cell, for the protoplast is dependent upon this gas for the performance of its vital functions, and particularly for the liberation of the energy which is demanded in the constructive processes.
The protoplast is uninucleate and vacuolate, and contains chromatophores of a brownish colour.
Of a cell or cells, the protoplast of which contains a single nucleus.