Control tube 8 is designed to verify that the patient's serum will
not lyse
the horse red blood cells.
The bacterial
cells lysed
within 30 minutes to form a clear suspension.
Lysed by sonication, 10 s on, 2 s off at 40 % amplitude for 8 min.
The selected cd4 cells are washed on a magnet tray and
then lysed
to release intracellular atp.
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