Do not keep the ligature longer than half an hour.
Apply a ligature above, not on the top of, the situation of the bite, twist the string tightly with a stick.
Then let the circulation return, and apply the ligature again.
The outer bark of each being removed, the two shoots are kept in contact by ligature until union is established, when the scion is completely severed from its original attachments.
A tight ligature of twine or wire answers the same end.
Apply the ligature, if possible, at the bleeding point, tying both ends of the cut vessel.
In any case do not keep the ligature on for more than an hour for fear of gangrene.
Welch produced oedema of the lungs experimentally by increasing the pressure in the pulmonary vessels by ligature of the aorta and its branches, but this raised the blood pressure only about one-tenth of an atmosphere, while in some of Loeb's experiments the osmotic pressure, due to retained metabolic products, was equal to over thirty atmospheres.
In large vessels it is usual to pass a ligature round the vessel and tie it with a reef-knot.
Haemorrhage has been classified as - (I) primary, occurring at the time of the injury; (2) reactionary, or within twenty-four hours of the accident, during the stage of reaction; (3) secondary, occurring at a later period and caused by faulty application of a ligature or septic condition of the wound.
This dilatation may be increased by local warmth, and poultices or fomentations are commonly applied to inflamed parts; recently suction apparatus has been used for the same purpose or ligature so as to cause venous stasis (Bier's treatment).