Bergh (for Lumbricus and Criodrilus), whose figures show a derivation of the entire nephridium from mesoblast, and an absence of any connexion between successive nephridia by any continuous band, epiblastic or mesoblastic. A midway position is taken up by Wilson, who asserts the mesoblastic formation of the funnel, but also asserts the presence of a continuous band of epiblast from which certainly the terminal vesicle of the nephridium, and doubtfully the glandular part of the tube is derived.
The proboscis is an invagination from the epiblast; the proboscidian sheath appears in the mesoblast, but is perhaps originally derived from the hypoblast.
The nerve ganglion is formed by an ingrowth of epiblast, and so are the pedal glands.
Moreover, the body cavity of the rotifers is a primitive archicoele; the persistent or accrescent cleft between epiblast and hypoblast, traversed by mesenchymal muscular bands.
It is based upon the fact that the histological differentiation of the epidermis of their root is that generally characteristic of Monocotyledons, whilst they have two cotyledons - the old view of the epiblast as a second cotyledon in Gramineae being adopted.
The embryo now consists of two layers of cells, epiblast and hypoblast, surrounding a cavity, the archenteron, which opens to the exterior by the orifice of invagination or blastopore.
The primordium of the neurochord (neural or medullary plate) referred to above becomes closed in from the surface by the overgrowth of surrounding epiblast, and its edges also bend up, meet, and finally fuse to form a tube, the medullary or neural tube.
An important fact to note is that the blastopore is included in this overgrowth of epiblast, so that the neural tube remains for some time in open communication with the archenteron by means of a posterior neurenteric canal.
The epiblast has been regarded as representing a second cotyledon, but this is a very doubtful interpretation.